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„Sanger-Sequencing“

Our sequencing service includes cycle sequencing, capillary electrophoresis and transfer of sequencing results via e-mail. All cycle sequencing reactions are automatically purified using CleanSeq® beads.

The separation of the sequencing reactions (fragment analysis [electrophoresis service] on request) is performed using an ABI3730 (48 capillaries)or 3130XL DNA sequencer (16 capillaries) with a guaranteed reading length of 700 bases.

PremixSingleRun €3,30

  • Sequencing of purified PCR products and plasmids
  • You mix DNA and primers with defined volume and concentration* in advance
  • No editing, no repetition

 

Recommended formats: 1.5 ml, 0.5 ml or 0.2 ml reaction tube (no screw cap! no parafilm!)
Other formats (96-wells, 8-strips) on request

PremixPrepaid €3,00

  • Performance and specification like “PremixSingleRun”.
  • Minimum purchase quantity 250 sequencings

 

Recommended formats: 1.5 ml, 0.5 ml or 0.2 ml reaction tube (no screw cap! no parafilm!)
Other formats (96-wells, 8-strips) on request

ComfortRun €3,90

  • Sequencing of purified PCR products and plasmids
  • You send DNA with defined volume and concentration*
  • Primer separately or selection from our primer list**
  • Repetition in case of limited quality possible after consultation
  • Surcharge for uncleaned PCRs (clean-up by Exo-SAP incubation): € 0,30

 

Recommended formats: 1.5 ml, 0.5 ml or 0.2 ml reaction tube (no screw cap! no parafilm!)
Other formats (96-wells, 8-strips) on request

Specifications

DNA length (bp) DNA concentration PremixSingleRun ComfortRun
PCR products

150-400

20ng/µl

8µl DNA plus 2µl (5pmol/µl) primer 12µl DNA

400-700

30ng/µl

700-1500

50ng/µl

>1500

80ng/µl

Plasmids >2000

100 ng/µl